"First Detection of PER-Type Extended-Spectrum  β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "

Rahimatou Yasmine Tiemtoré; Amana Mètuor-Dabiré; Theodora Mahoukèdè Zohoncon; Yasmine Aminata Bangré; Serge Sougue; Jacob Zongo; Jacques Simpore

doi:doi:10.11648/j.ijbbmb.20190401.12

"First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "

Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, Jacob Zongo, Jacques Simpore

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 4, Issue 1)

Received: 14 May 2019 Accepted: 17 June 2019 Published: 28 June 2019

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Abstract

Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 4, Issue 1)
DOI	10.11648/j.ijbbmb.20190401.12
Page(s)	7-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Broad-spectrum Beta-lactamases (ESBL), PER Gene, PCR

References

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Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, et al. (2019). "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". International Journal of Biochemistry, Biophysics & Molecular Biology, 4(1), 7-12. https://doi.org/10.11648/j.ijbbmb.20190401.12

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ACS Style

Rahimatou Yasmine Tiemtoré; Amana Mètuor-Dabiré; Theodora Mahoukèdè Zohoncon; Yasmine Aminata Bangré; Serge Sougue, et al. "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". Int. J. Biochem. Biophys. Mol. Biol. 2019, 4(1), 7-12. doi: 10.11648/j.ijbbmb.20190401.12

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AMA Style

Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, et al. "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". Int J Biochem Biophys Mol Biol. 2019;4(1):7-12. doi: 10.11648/j.ijbbmb.20190401.12

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@article{10.11648/j.ijbbmb.20190401.12,
  author = {Rahimatou Yasmine Tiemtoré and Amana Mètuor-Dabiré and Theodora Mahoukèdè Zohoncon and Yasmine Aminata Bangré and Serge Sougue and Jacob Zongo and Jacques Simpore},
  title = {"First Detection of PER-Type Extended-Spectrum  β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {4},
  number = {1},
  pages = {7-12},
  doi = {10.11648/j.ijbbmb.20190401.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20190401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20190401.12},
  abstract = {Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.},
 year = {2019}
}

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TY - JOUR
T1 - "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "
AU - Rahimatou Yasmine Tiemtoré
AU - Amana Mètuor-Dabiré
AU - Theodora Mahoukèdè Zohoncon
AU - Yasmine Aminata Bangré
AU - Serge Sougue
AU - Jacob Zongo
AU - Jacques Simpore
Y1 - 2019/06/28
PY - 2019
N1 - https://doi.org/10.11648/j.ijbbmb.20190401.12
DO - 10.11648/j.ijbbmb.20190401.12
T2 - International Journal of Biochemistry, Biophysics & Molecular Biology
JF - International Journal of Biochemistry, Biophysics & Molecular Biology
JO - International Journal of Biochemistry, Biophysics & Molecular Biology
SP - 7
EP - 12
PB - Science Publishing Group
SN - 2575-5862
UR - https://doi.org/10.11648/j.ijbbmb.20190401.12
AB - Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.
VL - 4
IS - 1
ER -

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Author Information

Rahimatou Yasmine Tiemtoré

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Amana Mètuor-Dabiré

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Theodora Mahoukèdè Zohoncon

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Yasmine Aminata Bangré

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Serge Sougue

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Jacob Zongo

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso
Jacques Simpore

Department of Biochemistry-Microbiology, University JOSEPH KI-ZERBO, Ouagadougou, Burkina Faso

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APA Style

Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, et al. (2019). "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". International Journal of Biochemistry, Biophysics & Molecular Biology, 4(1), 7-12. https://doi.org/10.11648/j.ijbbmb.20190401.12

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ACS Style

Rahimatou Yasmine Tiemtoré; Amana Mètuor-Dabiré; Theodora Mahoukèdè Zohoncon; Yasmine Aminata Bangré; Serge Sougue, et al. "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". Int. J. Biochem. Biophys. Mol. Biol. 2019, 4(1), 7-12. doi: 10.11648/j.ijbbmb.20190401.12

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AMA Style

Rahimatou Yasmine Tiemtoré, Amana Mètuor-Dabiré, Theodora Mahoukèdè Zohoncon, Yasmine Aminata Bangré, Serge Sougue, et al. "First Detection of PER-Type Extended-Spectrum β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso ". Int J Biochem Biophys Mol Biol. 2019;4(1):7-12. doi: 10.11648/j.ijbbmb.20190401.12

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@article{10.11648/j.ijbbmb.20190401.12,
  author = {Rahimatou Yasmine Tiemtoré and Amana Mètuor-Dabiré and Theodora Mahoukèdè Zohoncon and Yasmine Aminata Bangré and Serge Sougue and Jacob Zongo and Jacques Simpore},
  title = {"First Detection of PER-Type Extended-Spectrum  β-lactamases at Saint Camille Hospital Center of Ouagadougou, Burkina Faso "},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {4},
  number = {1},
  pages = {7-12},
  doi = {10.11648/j.ijbbmb.20190401.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20190401.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20190401.12},
  abstract = {Resistance to a wide variety of common antimicrobials is observed among clinical strains designated as extended-spectrum β-lactamase (ESBL) producers. They produce enzymatic proteins that effectively inactivate cephalosporins and aztreonam and are a serious global health problem that complicates treatment strategies. Many studies report a high prevalence of ESBL producers among Gram-negative bacilli. The purpose of this work was to identify of PER resistance gene in enterobacterial strains. Gram-negative bacilli resistant to at least one third-generation cephalosporin, Aztreonam or showing a synergistic image between amoxicillin + clavulanic acid and a third generation cephalosporin were isolated during an antibiogram. Antibiotic resistance was detected for the following antibiotics: Ceftriaxone, Cefotaxime, Ceftazidime and Aztreonam. A classical polymerase chain reaction (PCR) analysis of the β-lactamase PER (Pseudomonas Extended Resistance) gene was performed using specific primers in 60 ESBL-producing isolates. Among 250 strains of Gram negative bacilli collected, 60 strains (24%) showed resistance to antibiotics used. Stool samples are a major source of ESBL producers. The highest prevalence of resistant strains was observed in Escherichia coli with a rate of 35%. Among the producers of ESBL isolates, the presence of the PER gene was detected in the present study by up to 15% in 6 bacterial species. This study represents the first detection of the PER gene in Burkina Faso.},
 year = {2019}
}

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