Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of <i>Citrus Limon (L)</i>

Ayuba Yohanna Musa; Okoro Benedict Chidiogor; Abubakar Muhammad Nazif; Ilesanmi Esther

doi:doi:10.11648/j.ijbbmb.20170204.12

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Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)

Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 2, Issue 4)

Received: 22 August 2017 Accepted: 5 September 2017 Published: 30 September 2017

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Abstract

Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 2, Issue 4)
DOI	10.11648/j.ijbbmb.20170204.12
Page(s)	31-35
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Phytochemical, Antimicrobial, TLC, Staphylococcus Aureus, Candida Albicans, Bacillus Subtillis

References

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APA Style

Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther. (2017). Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). International Journal of Biochemistry, Biophysics & Molecular Biology, 2(4), 31-35. https://doi.org/10.11648/j.ijbbmb.20170204.12

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ACS Style

Ayuba Yohanna Musa; Okoro Benedict Chidiogor; Abubakar Muhammad Nazif; Ilesanmi Esther. Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). Int. J. Biochem. Biophys. Mol. Biol. 2017, 2(4), 31-35. doi: 10.11648/j.ijbbmb.20170204.12

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AMA Style

Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther. Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). Int J Biochem Biophys Mol Biol. 2017;2(4):31-35. doi: 10.11648/j.ijbbmb.20170204.12

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@article{10.11648/j.ijbbmb.20170204.12,
  author = {Ayuba Yohanna Musa and Okoro Benedict Chidiogor and Abubakar Muhammad Nazif and Ilesanmi Esther},
  title = {Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {2},
  number = {4},
  pages = {31-35},
  doi = {10.11648/j.ijbbmb.20170204.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20170204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20170204.12},
  abstract = {Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.},
 year = {2017}
}

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TY  - JOUR
T1  - Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)
AU  - Ayuba Yohanna Musa
AU  - Okoro Benedict Chidiogor
AU  - Abubakar Muhammad Nazif
AU  - Ilesanmi Esther
Y1  - 2017/09/30
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20170204.12
DO  - 10.11648/j.ijbbmb.20170204.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 31
EP  - 35
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20170204.12
AB  - Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.
VL  - 2
IS  - 4
ER  -

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Author Information

Ayuba Yohanna Musa

Department of Chemistry, Abubakar Tafawa Balewa University Bauchi, Bauchi, Nigeria
Okoro Benedict Chidiogor

Department of Microbiology, Godfrey Okoye University Emene, Enugu, Nigeria
Abubakar Muhammad Nazif

Department of BioTechnology, Modibbo Adama University of Technology Yola, Adamawa, Nigeria
Ilesanmi Esther

Department of Chemistry, University of Benin, Benin, Nigeria

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APA Style

Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther. (2017). Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). International Journal of Biochemistry, Biophysics & Molecular Biology, 2(4), 31-35. https://doi.org/10.11648/j.ijbbmb.20170204.12

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ACS Style

Ayuba Yohanna Musa; Okoro Benedict Chidiogor; Abubakar Muhammad Nazif; Ilesanmi Esther. Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). Int. J. Biochem. Biophys. Mol. Biol. 2017, 2(4), 31-35. doi: 10.11648/j.ijbbmb.20170204.12

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AMA Style

Ayuba Yohanna Musa, Okoro Benedict Chidiogor, Abubakar Muhammad Nazif, Ilesanmi Esther. Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L). Int J Biochem Biophys Mol Biol. 2017;2(4):31-35. doi: 10.11648/j.ijbbmb.20170204.12

Copy | Download

@article{10.11648/j.ijbbmb.20170204.12,
  author = {Ayuba Yohanna Musa and Okoro Benedict Chidiogor and Abubakar Muhammad Nazif and Ilesanmi Esther},
  title = {Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {2},
  number = {4},
  pages = {31-35},
  doi = {10.11648/j.ijbbmb.20170204.12},
  url = {https://doi.org/10.11648/j.ijbbmb.20170204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20170204.12},
  abstract = {Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.},
 year = {2017}
}

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TY  - JOUR
T1  - Phytochemical Constituents, Thin Layer Chromatography and Antimicrobial Activity of Methanol Extract of the Stem and Leave of Citrus Limon (L)
AU  - Ayuba Yohanna Musa
AU  - Okoro Benedict Chidiogor
AU  - Abubakar Muhammad Nazif
AU  - Ilesanmi Esther
Y1  - 2017/09/30
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20170204.12
DO  - 10.11648/j.ijbbmb.20170204.12
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 31
EP  - 35
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20170204.12
AB  - Citrus limon (L) leaf and stem used traditionally for the treatment of diseases and infections was phytochemically and antimicrobially screened. And also Thin layer Chromatography (TLC) was carried out on the crude methanol extracts. The phytoconstituents were qualitatively determined. The phytochemical screening of the crude methanol extract revealed the presence of saponins, terpenes, flavonoids, steroids, tannins, carbohydrates, anthraquinones, alkaloids, volatile oils and glycosides. The result of the antimicrobial screening of the crude methanol extract of the leaf showed potent activity against Staphylococcus aureus, candida albicans and bacillus subtillis. However, the crude methanol extract of the stem only showed activity against Staphylococcus aureus. TLC analysis of the of the crude methanol extract of the leaf showed three (3) spots while that of the stem revealed two (2) spots.
VL  - 2
IS  - 4
ER  -

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