Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with <i>Plasmodium falciparum</i> Malaria in Katsina State, Nigeria

Bello Ibrahim; Abdulsalami Mohammed Sani; Bulus Timothy; Mukhtar Gambo Lawal; Abdullahi Mohammed; Abdurrahman Siraj Abubakar; Ahmad Mohammad

doi:doi:10.11648/j.ijbbmb.20160102.13

Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria

Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, Abdurrahman Siraj Abubakar, Ahmad Mohammad

Published in International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 1, Issue 2)

Received: 18 November 2016 Accepted: 7 January 2017 Published: 3 February 2017

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Abstract

G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.

Published in	International Journal of Biochemistry, Biophysics & Molecular Biology (Volume 1, Issue 2)
DOI	10.11648/j.ijbbmb.20160102.13
Page(s)	46-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Glucose-6-phosphate Dehydrogenase, Plasmodium falciparum, Malaria, Children, Katsina State, Nigeria

References

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Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. (2017). Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. International Journal of Biochemistry, Biophysics & Molecular Biology, 1(2), 46-49. https://doi.org/10.11648/j.ijbbmb.20160102.13

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ACS Style

Bello Ibrahim; Abdulsalami Mohammed Sani; Bulus Timothy; Mukhtar Gambo Lawal; Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int. J. Biochem. Biophys. Mol. Biol. 2017, 1(2), 46-49. doi: 10.11648/j.ijbbmb.20160102.13

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AMA Style

Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int J Biochem Biophys Mol Biol. 2017;1(2):46-49. doi: 10.11648/j.ijbbmb.20160102.13

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@article{10.11648/j.ijbbmb.20160102.13,
  author = {Bello Ibrahim and Abdulsalami Mohammed Sani and Bulus Timothy and Mukhtar Gambo Lawal and Abdullahi Mohammed and Abdurrahman Siraj Abubakar and Ahmad Mohammad},
  title = {Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {1},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ijbbmb.20160102.13},
  url = {https://doi.org/10.11648/j.ijbbmb.20160102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20160102.13},
  abstract = {G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.},
 year = {2017}
}

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TY  - JOUR
T1  - Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria
AU  - Bello Ibrahim
AU  - Abdulsalami Mohammed Sani
AU  - Bulus Timothy
AU  - Mukhtar Gambo Lawal
AU  - Abdullahi Mohammed
AU  - Abdurrahman Siraj Abubakar
AU  - Ahmad Mohammad
Y1  - 2017/02/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20160102.13
DO  - 10.11648/j.ijbbmb.20160102.13
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 46
EP  - 49
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20160102.13
AB  - G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.
VL  - 1
IS  - 2
ER  -

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Author Information

Bello Ibrahim

Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Abdulsalami Mohammed Sani

Department of Biological Sciences, Nigerian Defence Academy, Kaduna, Nigeria
Bulus Timothy

Department of Biochemistry, Kaduna State University, Kaduna, Nigeria
Mukhtar Gambo Lawal

Department of Microbiology, Umaru Musa Yar’adua University, Katsina, Nigeria
Abdullahi Mohammed

Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Abdurrahman Siraj Abubakar

Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria
Ahmad Mohammad

Bioresources Development Centre Katsina, National Biotechnology Development Agency, Abuja, Nigeria

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APA Style

Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. (2017). Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. International Journal of Biochemistry, Biophysics & Molecular Biology, 1(2), 46-49. https://doi.org/10.11648/j.ijbbmb.20160102.13

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ACS Style

Bello Ibrahim; Abdulsalami Mohammed Sani; Bulus Timothy; Mukhtar Gambo Lawal; Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int. J. Biochem. Biophys. Mol. Biol. 2017, 1(2), 46-49. doi: 10.11648/j.ijbbmb.20160102.13

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AMA Style

Bello Ibrahim, Abdulsalami Mohammed Sani, Bulus Timothy, Mukhtar Gambo Lawal, Abdullahi Mohammed, et al. Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria. Int J Biochem Biophys Mol Biol. 2017;1(2):46-49. doi: 10.11648/j.ijbbmb.20160102.13

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@article{10.11648/j.ijbbmb.20160102.13,
  author = {Bello Ibrahim and Abdulsalami Mohammed Sani and Bulus Timothy and Mukhtar Gambo Lawal and Abdullahi Mohammed and Abdurrahman Siraj Abubakar and Ahmad Mohammad},
  title = {Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria},
  journal = {International Journal of Biochemistry, Biophysics & Molecular Biology},
  volume = {1},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ijbbmb.20160102.13},
  url = {https://doi.org/10.11648/j.ijbbmb.20160102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbbmb.20160102.13},
  abstract = {G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.},
 year = {2017}
}

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TY  - JOUR
T1  - Molecular Screening of Glucose-6-Phosphate Dehydrogenase Among Deficient Children Aged 0-5 Years with Plasmodium falciparum Malaria in Katsina State, Nigeria
AU  - Bello Ibrahim
AU  - Abdulsalami Mohammed Sani
AU  - Bulus Timothy
AU  - Mukhtar Gambo Lawal
AU  - Abdullahi Mohammed
AU  - Abdurrahman Siraj Abubakar
AU  - Ahmad Mohammad
Y1  - 2017/02/03
PY  - 2017
N1  - https://doi.org/10.11648/j.ijbbmb.20160102.13
DO  - 10.11648/j.ijbbmb.20160102.13
T2  - International Journal of Biochemistry, Biophysics & Molecular Biology
JF  - International Journal of Biochemistry, Biophysics & Molecular Biology
JO  - International Journal of Biochemistry, Biophysics & Molecular Biology
SP  - 46
EP  - 49
PB  - Science Publishing Group
SN  - 2575-5862
UR  - https://doi.org/10.11648/j.ijbbmb.20160102.13
AB  - G6PD deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas. The aim of this study is to molecularly screen G6PD enzyme among deficient children (aged 0-5 years) with Plasmodium falciparum malaria in Katsina State, North-western Nigeria, and a region with no any molecular information on G6PD enzyme. A total of 200 blood samples were collected from children with Plasmodium falciparum malaria attending six selected hospitals located across the three senatorial zones of the state from March 2015 to May 2015. G6PD deficiency was detected qualitatively using G6PD screening test. Polymerase Chain Reaction (PCR) of 7 samples (6 deficient and 1 control) shows the presence of G202A mutation in all the samples. The nucleotides sequence obtained from sequencing reaction of one deficient and one control samples are 97% homologous to other G6PD genes of different strains. This study has indicated a high prevalence of G6PD deficiency among the study population. Based on the results obtained, there is a need for the routine screening of children for G6PD deficiency in our environment and training of paediatricians in order to avoid cases of drug-induced anaemia, particularly in the treatment of malaria.
VL  - 1
IS  - 2
ER  -

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