Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan

Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier

doi:doi:10.11648/j.crj.20210902.12

| Peer-Reviewed

Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier

Published in Cancer Research Journal (Volume 9, Issue 2)

Received: 31 May 2020 Accepted: 4 July 2020 Published: 8 May 2021

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.

Published in	Cancer Research Journal (Volume 9, Issue 2)
DOI	10.11648/j.crj.20210902.12
Page(s)	92-97
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Breast Cancer, Risk Factors, Sudan, VIM Gene

References

[1]	Susan L and Paul C. (1998); Epidemiology in practice: Case-Control Studies. Community Eye Health. 11 (28): 57-58.
[2]	Siegel RL, Miller KD and Jemal A. (2015): Cancer statistics. CA Cancer J Clin; (65): 5-29.
[3]	Fitzmaurice C, Dicker D, Pain A, Hamavid H, Moradi-Lakeh M, MacIntyre MF. (2013). The global burden of cancer. JAMA Oncol. 2015; (1): 505-527.
[4]	Bray Fand Jemal A, Grey N. (2012). Global cancer transitions according to the Human Development Index (2008-2030): A population-based study. Lancet Oncol; (13): 790-801.
[5]	Jamison, D. T., J. G. Breman, A. R. Measham, G. Alleyne, M. Claeson, D. B. Evans (2006). Disease control priorities in developing countries. World Bank, Washington, DC.
[6]	Intisar E. Saeed, Hsin-Yi Weng, Kamal H Mohamed & Sulma I. Mohammed. (2014). Cancer incidence in Khartoum, Sudan: first results from the Cancer Registry, 2009–2010. Cancer Medicine; 3 (4): 1075-1084.
[7]	American Cancer Society. Breast Cancer Facts & Figures 2017-2018. Atlanta: American Cancer Society, Inc. 2017.
[8]	Stacey A. Missmer, A. Heather Eliassen, Robert L. Barbieri, Susan E. Hankinson (2004). Endogenous Estrogen, Androgen and Progesterone Concentrations and Breast Cancer Risk Among Postmenopausal Women, JNCI; 96 (24); 1856–1865.
[9]	Lip worth L, Bailey LR, Trichopoulos D. (2000) History of Breast-Feeding in Relation to Breast Cancer Risk: a Review of the Epidemiologic Literature. J Natl Cancer Inst; 92 (11): 302-312.
[10]	Jatoi I, Anderson WF, Rao SR, Devesa SS. (2005) Breast cancer trends among black and white women in the United States. J. Clin Oncol; 23 (31): 7836–7841.
[11]	Cheng F, Eriksson J. E. (2017) Intermediate Filaments and the Regulation of Cell Motility during Regeneration and Wound Healing. Cold Spring Harb. Perspect. Biol; (9) 220-46.
[12]	Dauphin M, Barbe C, Lemaire S, Nawrocki-Raby B, Lagonotte E, Delepine G, Birembaut P, Gilles C, Polette M. (2013). Vimentin expression predicts the occurrence of metastases in non small cell lung carcinomas. Lung Cancer; 81: 117–122.
[13]	Satelli A. and Li S. (. 2011). Vimentin in cancer and its potential as a molecular target for cancer therapy. Cell Mol Life Sci; 68 (18): 3033–3046.
[14]	Http\www.finchTV.
[15]	TA Hall - Nucleic acids symposium series (1999) - jwbrown.mbio.ncsu.edu.
[16]	Mohamed E. M. Saeed1, Jingming Cao1, Babikir Fadul, Onat Kadioglu, Hassan E. Khalid, Zahir Yassin, Siddig M. Mustafa, Elfatih Saeed And Thomas Efferth (2016) A Five-Year Survey Of Cancer Prevalence In Sudan. Anticancer Research; (36): 279-286.
[17]	Jatoi I, Anderson WF, Rao SR, Devesa SS (2005). Breast cancer trends among black and white women in the United States. J Clin Oncol; 23 (31): 7836–7841.
[18]	Bowen RL, Duffy SW, Ryan DA, Hart IR, Jones JL.(2008) Early onset of breast cancer in a group of British black women. Br J Cancer; 98 (2): 277–281.
[19]	Yu ZG, Jia CX, Geng CZ, Tang JH, Zhang J, Liu LY. (2012) Risk factors related to female breast cancer in regions of Northeast China: a 1:3 matched case-control population-based study. Chin Med J (Engl); 125: 733-40.
[20]	Kharazmi E, Fallah M, Sundquist K, Hemminki K. (2012) Familial risk of early and late onset cancer: nationwide prospective cohort study. BMJ; 345 (10): 1136-8076.
[21]	Alexander K and Andrea SP. (2009) Established and Suspected Risk Factors in Breast Cancer Etiology. Breast Care; 4 (2): 82-87.
[22]	Ibrahim G. Alghamdi ab, Issam I. Hussaina, Mohamed S. Alghamdic, Mohamed A. El-Sheemyd.(2015) Early marriage is a potential risk factor for female breast cancer in the Eastern Region of Saudi Arabia. American Journal of Research Communication; 3 (7).
[23]	Aizer AA, Chen M-H, McCarthy EP. (2013) Marital status and survival in patients with cancer. J Clin Oncol; (31): 3869-76.
[24]	Britt K, Ashworth A, Smalley M. (2007) Pregnancy and the risk of breast cancer. Endocrine-Related Cancer; (14): 907-33.
[25]	Babita NK, Singh M, Malik JS. (2014)Breastfeeding reduces breast cancer risk: a case-control study in north India. Int. J Prev. Med; 791 (5).
[26]	Elima Jedy-Agba, Valerie McCormack, Clement Adebamowo and Isabel dos-Santos-Silva. (2016)Stage at diagnosis of breast cancer in sub-Saharan Africa: a systematic review and meta-analysis. Lancet Glob Health; 4 (12): e923–e935.
[27]	Collaborative Group on Hormonal Factors in Breast Cancer. Menarche, menopause, and breast cancer risk: individual participant meta-analysis, including 118,964 women with breast cancer from 117 epidemiological studies. Lancet Oncol. 2012; (13): 1141-1151.
[28]	Noh H, Yan J, Hong S, Kong LY, Gabrusiewicz, K, Xia X, Heimberger A. B, Li S. (2016)Discovery of cell surface vimentin targeting mAb for direct disruption of GBM tumor initiating cells. Onco. Target; (7): 72021–72032.
[29]	Kokkinos MI, Wafai R, Wong MK, Newgreen DF, Thompson EW, Waltham M. (2007)Vimentin and epithelial-mesenchymal transition in human breast cancer--observations in vitro and in vivo. Cells Tissues Organs; 185: 191–203.
[30]	GillesC, Polette M, Zahm J. M, Tournier J. M, Volders L, Foidart J. M, Birembaut P. (1999)Vimentin contributes to human mammary epithelial cell migration. J. Cell Sci.; 112 (24): 4615–4625.
[31]	Lahat G, Zhu Q S, Huang K L, Wang S, Bolshakov S, Liu J, Torres K, Langley RR, Lazar AJ, Hung M C.(2010) Vimentin Is a Novel Anti-Cancer Therapeutic Target; Insights from In Vitro and In Vivo Mice Xenograft Studies. PLoS ONE; (5): 101-105.

Cite This Article

Plain Text BibTeX RIS

APA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. (2021). Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Research Journal, 9(2), 92-97. https://doi.org/10.11648/j.crj.20210902.12

Copy | Download

ACS Style

Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res. J. 2021, 9(2), 92-97. doi: 10.11648/j.crj.20210902.12

Copy | Download

AMA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res J. 2021;9(2):92-97. doi: 10.11648/j.crj.20210902.12

Copy | Download

@article{10.11648/j.crj.20210902.12,
  author = {Hala Abdalgader Khairalseed Abas and Hind Abdelaziz Elnasri and Mona Abdelrahman Mohmed Khaier},
  title = {Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan},
  journal = {Cancer Research Journal},
  volume = {9},
  number = {2},
  pages = {92-97},
  doi = {10.11648/j.crj.20210902.12},
  url = {https://doi.org/10.11648/j.crj.20210902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20210902.12},
  abstract = {Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.},
 year = {2021}
}

Copy | Download

TY - JOUR
T1 - Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan
AU - Hala Abdalgader Khairalseed Abas
AU - Hind Abdelaziz Elnasri
AU - Mona Abdelrahman Mohmed Khaier
Y1 - 2021/05/08
PY - 2021
N1 - https://doi.org/10.11648/j.crj.20210902.12
DO - 10.11648/j.crj.20210902.12
T2 - Cancer Research Journal
JF - Cancer Research Journal
JO - Cancer Research Journal
SP - 92
EP - 97
PB - Science Publishing Group
SN - 2330-8214
UR - https://doi.org/10.11648/j.crj.20210902.12
AB - Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.
VL - 9
IS - 2
ER -

Copy | Download

Author Information

Hala Abdalgader Khairalseed Abas

Center of Medical Research, Faculty of Medicine, Al-Neelain University, Khartoum, Sudan
Hind Abdelaziz Elnasri

Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
Mona Abdelrahman Mohmed Khaier

Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. (2021). Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Research Journal, 9(2), 92-97. https://doi.org/10.11648/j.crj.20210902.12

Copy | Download

ACS Style

Hala Abdalgader Khairalseed Abas; Hind Abdelaziz Elnasri; Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res. J. 2021, 9(2), 92-97. doi: 10.11648/j.crj.20210902.12

Copy | Download

AMA Style

Hala Abdalgader Khairalseed Abas, Hind Abdelaziz Elnasri, Mona Abdelrahman Mohmed Khaier. Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan. Cancer Res J. 2021;9(2):92-97. doi: 10.11648/j.crj.20210902.12

Copy | Download

@article{10.11648/j.crj.20210902.12,
  author = {Hala Abdalgader Khairalseed Abas and Hind Abdelaziz Elnasri and Mona Abdelrahman Mohmed Khaier},
  title = {Risk Factors and Molecular Study of Vimentin Gene (VIM), Associated with Female Breast Cancer in Khartoum, Sudan},
  journal = {Cancer Research Journal},
  volume = {9},
  number = {2},
  pages = {92-97},
  doi = {10.11648/j.crj.20210902.12},
  url = {https://doi.org/10.11648/j.crj.20210902.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.crj.20210902.12},
  abstract = {Background: The aim of this study was to investigate the possible risk factors and mutations in VIM gene among Sudanese’s breast cancer women in Khartoum State. Methods: This case-control study involved 45 patients with breast cancer and 45controls. It was conducted across three hospitals and two laboratories in Khartoum State. A structural questionnaire was used to obtain data regarding age, family history, menarche, marriage, menopause, pregnancy, nulliparous and parous women, breast-feeding, use of fertility or contraceptive drugs and grade of the disease. DNA from patient and control tissues was extracted using extraction kits. PCR was conducted to amplify VIM gene using specific primers. PCR products were sequenced in order to detect the mutation in VIM gene. Data was analyzed using Pearson’s Chi-square tests to identify risk factors associated with breast cancer. Results: The study showed that the main risk factors associated with breast cancer were family history with first degree relative, menarche, irregularity of menarche, reproductive factors such as pregnancy, breast-feeding and nulliparous. DNA sequencing revealed no mutations in VIM gene associated with breast cancer in Sudanese women in Khartoum State. Conclusion: The association of other risk factors such as menopausal status, age of menopausal, oral contraceptive birth control and fertility hormones needs more illumination and further work. Other genes associated with breast cancer can be investigated.},
 year = {2021}
}

Copy | Download