Present investigation deals with the synthesis of silver nanoparticles (AgNPs) from Lycopersicon esculentum L. through simple and eco-friendly method and validation the capacity of nanoparticles to inhibit the virulence gene expression in Candida albicans. The nanoparticles thus obtained from Lycopersicon esculentum L. have been analysed and characterised by Scanning Electron Microscopy (SEM), UV-Vis spectrophotometer, X-ray diffraction (XRD) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. The average diameter of the AgNPs, whose morphology has been determined by SEM, was found to be 9.58 to 72.69 nm. The UV-vis spectrophotometer show peak located of silver nanoparticles at 340 nm. X-ray diffraction analysis also showed functional structure and pattern of silver nanoparticles. The FT-IR spectra indicated the role of different functional groups of reducing agent and silver nanoparticles. AgNPs at concentrations of 15 and 25% significantly downed expression of Sap1, LIP1 and Kex2, but had no effect on the expression of CDR1 gene. The findings of current study showed that tomato extract could be used as a green chemistry approach to produce AgNPs. It downed expression of Sap1, LIP1 and Kex2 genes, whereas had no effect on the expression of CDR1 gene, that it appears needs higher concentrations of AgNPs to inhibit its gene expression.
| Published in | American Journal of Nanosciences (Volume 6, Issue 4) |
| DOI | 10.11648/j.ajn.20200604.12 |
| Page(s) | 34-44 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Lycopersicon Esculentum, Agnanoparticles, Candida albicans, Virulence Genes Sap1, LIP1, Kex2, CDR1
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APA Style
Muhsen Mohamed Faraj, Kamil Mutashar Al-Jobori. (2021). Expression Analysis of the Candida albicans KEX2, SAP1, CDR1 and LIP1genes Influenced by Biosynthesized Silver Nanoparticles. American Journal of Nanosciences, 6(4), 34-44. https://doi.org/10.11648/j.ajn.20200604.12
ACS Style
Muhsen Mohamed Faraj; Kamil Mutashar Al-Jobori. Expression Analysis of the Candida albicans KEX2, SAP1, CDR1 and LIP1genes Influenced by Biosynthesized Silver Nanoparticles. Am. J. Nanosci. 2021, 6(4), 34-44. doi: 10.11648/j.ajn.20200604.12
AMA Style
Muhsen Mohamed Faraj, Kamil Mutashar Al-Jobori. Expression Analysis of the Candida albicans KEX2, SAP1, CDR1 and LIP1genes Influenced by Biosynthesized Silver Nanoparticles. Am J Nanosci. 2021;6(4):34-44. doi: 10.11648/j.ajn.20200604.12
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Download@article{10.11648/j.ajn.20200604.12,
author = {Muhsen Mohamed Faraj and Kamil Mutashar Al-Jobori},
title = {Expression Analysis of the Candida albicans KEX2, SAP1, CDR1 and LIP1genes Influenced by Biosynthesized Silver Nanoparticles},
journal = {American Journal of Nanosciences},
volume = {6},
number = {4},
pages = {34-44},
doi = {10.11648/j.ajn.20200604.12},
url = {https://doi.org/10.11648/j.ajn.20200604.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajn.20200604.12},
abstract = {Present investigation deals with the synthesis of silver nanoparticles (AgNPs) from Lycopersicon esculentum L. through simple and eco-friendly method and validation the capacity of nanoparticles to inhibit the virulence gene expression in Candida albicans. The nanoparticles thus obtained from Lycopersicon esculentum L. have been analysed and characterised by Scanning Electron Microscopy (SEM), UV-Vis spectrophotometer, X-ray diffraction (XRD) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. The average diameter of the AgNPs, whose morphology has been determined by SEM, was found to be 9.58 to 72.69 nm. The UV-vis spectrophotometer show peak located of silver nanoparticles at 340 nm. X-ray diffraction analysis also showed functional structure and pattern of silver nanoparticles. The FT-IR spectra indicated the role of different functional groups of reducing agent and silver nanoparticles. AgNPs at concentrations of 15 and 25% significantly downed expression of Sap1, LIP1 and Kex2, but had no effect on the expression of CDR1 gene. The findings of current study showed that tomato extract could be used as a green chemistry approach to produce AgNPs. It downed expression of Sap1, LIP1 and Kex2 genes, whereas had no effect on the expression of CDR1 gene, that it appears needs higher concentrations of AgNPs to inhibit its gene expression.},
year = {2021}
}
TY - JOUR T1 - Expression Analysis of the Candida albicans KEX2, SAP1, CDR1 and LIP1genes Influenced by Biosynthesized Silver Nanoparticles AU - Muhsen Mohamed Faraj AU - Kamil Mutashar Al-Jobori Y1 - 2021/01/18 PY - 2021 N1 - https://doi.org/10.11648/j.ajn.20200604.12 DO - 10.11648/j.ajn.20200604.12 T2 - American Journal of Nanosciences JF - American Journal of Nanosciences JO - American Journal of Nanosciences SP - 34 EP - 44 PB - Science Publishing Group SN - 2575-4858 UR - https://doi.org/10.11648/j.ajn.20200604.12 AB - Present investigation deals with the synthesis of silver nanoparticles (AgNPs) from Lycopersicon esculentum L. through simple and eco-friendly method and validation the capacity of nanoparticles to inhibit the virulence gene expression in Candida albicans. The nanoparticles thus obtained from Lycopersicon esculentum L. have been analysed and characterised by Scanning Electron Microscopy (SEM), UV-Vis spectrophotometer, X-ray diffraction (XRD) and Fourier Transform Infra-red Spectroscopy (FTIR) techniques. The average diameter of the AgNPs, whose morphology has been determined by SEM, was found to be 9.58 to 72.69 nm. The UV-vis spectrophotometer show peak located of silver nanoparticles at 340 nm. X-ray diffraction analysis also showed functional structure and pattern of silver nanoparticles. The FT-IR spectra indicated the role of different functional groups of reducing agent and silver nanoparticles. AgNPs at concentrations of 15 and 25% significantly downed expression of Sap1, LIP1 and Kex2, but had no effect on the expression of CDR1 gene. The findings of current study showed that tomato extract could be used as a green chemistry approach to produce AgNPs. It downed expression of Sap1, LIP1 and Kex2 genes, whereas had no effect on the expression of CDR1 gene, that it appears needs higher concentrations of AgNPs to inhibit its gene expression. VL - 6 IS - 4 ER -
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