Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria

Barivule Girigiri; Caroline Nchedu Ariole; Herbert Okechukwu Stanley

doi:doi:10.11648/j.ajn.20190504.11

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Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria

Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley

Published in American Journal of Nanosciences (Volume 5, Issue 4)

Received: 24 October 2019 Accepted: 14 November 2019 Published: 19 November 2019

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Abstract

This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.

Published in	American Journal of Nanosciences (Volume 5, Issue 4)
DOI	10.11648/j.ajn.20190504.11
Page(s)	27-38
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Bioremediation, Nitrogen-fixing Bacteria, Phosphate-solubilizing Bacteria, Biofertilizer, Crude Oil Polluted Soil

References

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APA Style

Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. (2019). Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. American Journal of Nanosciences, 5(4), 27-38. https://doi.org/10.11648/j.ajn.20190504.11

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ACS Style

Barivule Girigiri; Caroline Nchedu Ariole; Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am. J. Nanosci. 2019, 5(4), 27-38. doi: 10.11648/j.ajn.20190504.11

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AMA Style

Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am J Nanosci. 2019;5(4):27-38. doi: 10.11648/j.ajn.20190504.11

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@article{10.11648/j.ajn.20190504.11,
  author = {Barivule Girigiri and Caroline Nchedu Ariole and Herbert Okechukwu Stanley},
  title = {Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria},
  journal = {American Journal of Nanosciences},
  volume = {5},
  number = {4},
  pages = {27-38},
  doi = {10.11648/j.ajn.20190504.11},
  url = {https://doi.org/10.11648/j.ajn.20190504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajn.20190504.11},
  abstract = {This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.},
 year = {2019}
}

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TY  - JOUR
T1  - Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria
AU  - Barivule Girigiri
AU  - Caroline Nchedu Ariole
AU  - Herbert Okechukwu Stanley
Y1  - 2019/11/19
PY  - 2019
N1  - https://doi.org/10.11648/j.ajn.20190504.11
DO  - 10.11648/j.ajn.20190504.11
T2  - American Journal of Nanosciences
JF  - American Journal of Nanosciences
JO  - American Journal of Nanosciences
SP  - 27
EP  - 38
PB  - Science Publishing Group
SN  - 2575-4858
UR  - https://doi.org/10.11648/j.ajn.20190504.11
AB  - This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.
VL  - 5
IS  - 4
ER  -

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Author Information

Barivule Girigiri

Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria
Caroline Nchedu Ariole

Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria
Herbert Okechukwu Stanley

Department of Microbiology, Faculty of Science, University of Port Harcourt, Port Harcourt, Nigeria

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Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. (2019). Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. American Journal of Nanosciences, 5(4), 27-38. https://doi.org/10.11648/j.ajn.20190504.11

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ACS Style

Barivule Girigiri; Caroline Nchedu Ariole; Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am. J. Nanosci. 2019, 5(4), 27-38. doi: 10.11648/j.ajn.20190504.11

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AMA Style

Barivule Girigiri, Caroline Nchedu Ariole, Herbert Okechukwu Stanley. Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria. Am J Nanosci. 2019;5(4):27-38. doi: 10.11648/j.ajn.20190504.11

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@article{10.11648/j.ajn.20190504.11,
  author = {Barivule Girigiri and Caroline Nchedu Ariole and Herbert Okechukwu Stanley},
  title = {Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria},
  journal = {American Journal of Nanosciences},
  volume = {5},
  number = {4},
  pages = {27-38},
  doi = {10.11648/j.ajn.20190504.11},
  url = {https://doi.org/10.11648/j.ajn.20190504.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajn.20190504.11},
  abstract = {This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.},
 year = {2019}
}

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TY  - JOUR
T1  - Bioremediation of Crude Oil Polluted Soil Using Biofertilizer from Nitrogen-fixing and Phosphate-solubilizing Bacteria
AU  - Barivule Girigiri
AU  - Caroline Nchedu Ariole
AU  - Herbert Okechukwu Stanley
Y1  - 2019/11/19
PY  - 2019
N1  - https://doi.org/10.11648/j.ajn.20190504.11
DO  - 10.11648/j.ajn.20190504.11
T2  - American Journal of Nanosciences
JF  - American Journal of Nanosciences
JO  - American Journal of Nanosciences
SP  - 27
EP  - 38
PB  - Science Publishing Group
SN  - 2575-4858
UR  - https://doi.org/10.11648/j.ajn.20190504.11
AB  - This study examined bioremediation of crude oil polluted soil (PS) stimulated with nitrogen-fixing bacteria (NFB) and phosphate-solubilizing bacteria (PSB). Five set-ups designated as A (500 g PS + 50 g NFB); B (500 g PS + 50 g PSB); C (500 g PS + 50 g NFB+PSB); D (500 g PS + 50 g NPK); and E (500 g PS only: control) were designed. Total petroleum hydrocarbons (TPHs) were monitored for 4 weeks. Toxicity of the biofertilizer on maize plant was determined. The combination of the biofertilizers with normal soil for the ecotoxicity testing was in the following ratios: 100:0, 75:25, 50:50, 25:75, and 0:100. The NFB were classified as Azotobacter sp. and Rhizobium sp. while the PSB identifies as Pseudomonas and Bacillus using their 16S rRNA gene sequences and deposited in GenBank under the accession numbers MN134485.1-MN134488.1. After 28 days study, TPH reductions were 97.8%, 97.5%, 94.3%, 92.1%, and 34.6% in NFB, NFB+PSB, PSB, NPK treatments, and control. There was significant difference (P<0.05) between the set-ups when compared to the control. For toxicity testing, the 25:75 concentration in all treatment set-ups best supported plant growth. It was concluded that biofertilizer is effective in remediating oil contaminated soil and in improving soil fertility.
VL  - 5
IS  - 4
ER  -

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