Industrial Propagation of <i>Coffea</i> SP. by Bioreactor Technique

Tran Van Minh

doi:doi:10.11648/j.ajaf.20231102.14

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Industrial Propagation of Coffea SP. by Bioreactor Technique

Tran Van Minh

Published in American Journal of Agriculture and Forestry (Volume 11, Issue 2)

Received: 8 March 2023 Accepted: 1 April 2023 Published: 13 April 2023

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Abstract

Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m²/s, and 30rpm. Micropropagation of coffea sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.

Published in	American Journal of Agriculture and Forestry (Volume 11, Issue 2)
DOI	10.11648/j.ajaf.20231102.14
Page(s)	67-73
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Coffea sp, Micropropagation, Somatic Embryogenesis Suspension, Immersion-Bioreactor, Bioreactor Technique

References

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[8]	Park, S. Y., Murthy, H. N., & Paek, K. Y. (2003). PLB induction and subsequent plant regeneration from root tip cultures of Doritaenopsis. Plant Sci, 164, 919-923.
[9]	Shakti, M., Goel, M. K., Kukreja, A. K., & Mishra, B. N. (2007). Efficiency of liquid culture systems over conventional micropropagation: A progress towards commercialization. African J Biotechnology, 13, 1484-1492.
[10]	Son, S. H., Choi, S. M., Yun, S. R., Kwon, U. W., Lee, Y. H., & Paek, K. Y. (1999). Large scale culture of plant cell and tissue by bioreactor system. J Plant Biotech, 1, 1-7.
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[14]	Etienne H. & Berthouly M. (2002). Temporary immersion systems in plant micropropagation. Plant Cell, Tissue and Organ Culture, 69, 215-231.
[15]	Niemenak N., Saar-Surminski K., Rohsius C., Ndoumou D. O. & Lieberei R. (2008). Regeneration of somatic embryos in Theobroma cacao L. in temporary immersion bioreactor and analyses of free amino acids in different tissues. Plant Cell Report, 27, 667-676.

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APA Style

Tran Van Minh. (2023). Industrial Propagation of Coffea SP. by Bioreactor Technique. American Journal of Agriculture and Forestry, 11(2), 67-73. https://doi.org/10.11648/j.ajaf.20231102.14

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ACS Style

Tran Van Minh. Industrial Propagation of Coffea SP. by Bioreactor Technique. Am. J. Agric. For. 2023, 11(2), 67-73. doi: 10.11648/j.ajaf.20231102.14

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Tran Van Minh. Industrial Propagation of Coffea SP. by Bioreactor Technique. Am J Agric For. 2023;11(2):67-73. doi: 10.11648/j.ajaf.20231102.14

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@article{10.11648/j.ajaf.20231102.14,
  author = {Tran Van Minh},
  title = {Industrial Propagation of Coffea SP. by Bioreactor Technique},
  journal = {American Journal of Agriculture and Forestry},
  volume = {11},
  number = {2},
  pages = {67-73},
  doi = {10.11648/j.ajaf.20231102.14},
  url = {https://doi.org/10.11648/j.ajaf.20231102.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20231102.14},
  abstract = {Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m2/s, and 30rpm. Micropropagation of coffea sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.},
 year = {2023}
}

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TY  - JOUR
T1  - Industrial Propagation of Coffea SP. by Bioreactor Technique
AU  - Tran Van Minh
Y1  - 2023/04/13
PY  - 2023
N1  - https://doi.org/10.11648/j.ajaf.20231102.14
DO  - 10.11648/j.ajaf.20231102.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 67
EP  - 73
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20231102.14
AB  - Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m2/s, and 30rpm. Micropropagation of coffea sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.
VL  - 11
IS  - 2
ER  -

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Author Information

Tran Van Minh

Scool of Biotechnology, International University, Vietnam National University Ho Chi Minh City, Ho Chi Minh City, Vietnam

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APA Style

Tran Van Minh. (2023). Industrial Propagation of Coffea SP. by Bioreactor Technique. American Journal of Agriculture and Forestry, 11(2), 67-73. https://doi.org/10.11648/j.ajaf.20231102.14

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ACS Style

Tran Van Minh. Industrial Propagation of Coffea SP. by Bioreactor Technique. Am. J. Agric. For. 2023, 11(2), 67-73. doi: 10.11648/j.ajaf.20231102.14

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AMA Style

Tran Van Minh. Industrial Propagation of Coffea SP. by Bioreactor Technique. Am J Agric For. 2023;11(2):67-73. doi: 10.11648/j.ajaf.20231102.14

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@article{10.11648/j.ajaf.20231102.14,
  author = {Tran Van Minh},
  title = {Industrial Propagation of Coffea SP. by Bioreactor Technique},
  journal = {American Journal of Agriculture and Forestry},
  volume = {11},
  number = {2},
  pages = {67-73},
  doi = {10.11648/j.ajaf.20231102.14},
  url = {https://doi.org/10.11648/j.ajaf.20231102.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20231102.14},
  abstract = {Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m2/s, and 30rpm. Micropropagation of coffea sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.},
 year = {2023}
}

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TY  - JOUR
T1  - Industrial Propagation of Coffea SP. by Bioreactor Technique
AU  - Tran Van Minh
Y1  - 2023/04/13
PY  - 2023
N1  - https://doi.org/10.11648/j.ajaf.20231102.14
DO  - 10.11648/j.ajaf.20231102.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 67
EP  - 73
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20231102.14
AB  - Study is carried out at National Key Lab of Plant Cell Biotechnology, Ho Chi Minh City, Vietnam. Vietnam has planning to re-culture of coffea plant in area of over 40.000ha in South of Vietnam. Good quality of seedlings have a big demand. Using bioreactor technique is the first method to resolve the lack of seedlings. Stem, leaves of in vitro plantlets were used as planting materials. Somatic embryo callus was initiated on medium supplemented with MS+ BA (0.1mg/l) + 2iP (2mg/l) + IBA (2mg/l). Somatic cell suspension was created and proliferated on medium MS + kinetin (1mg/l) + BA (0.1mg/l) + 2iP (2mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS + kinetin (1mg/l) + BA (4mg/l). In vitro shoots of coffee were regeneration on the medium MS + kinetin (1mg/l) + BA (4mg/l) + Adenin sulfate (40mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/6hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favoured at 26±2°C, 11.1-22.2μmol/m2/s, and 30rpm. Micropropagation of coffea sp. by bioreactor technique was set up to produce 25,260 plantlets per liter of somatic embryogenesis suspension.
VL  - 11
IS  - 2
ER  -

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