Backgroud: Dendrobium sp. are production by multishoot system and it is required high of labor, energy, cost, large area. Objectives: there are needs of a new production system by using of plant cell culture techniques. Methods: manipulation of bioreactor techniques was effective ways to resolve the chalenges. Protocorm like bodies were used as planting materials. Somatic embryo callus was initiated on medium MS supplemented with 2.4D (0,3mg/l) + CW (30%). Somatic cell suspension was cultured for initiation and for proliferation and on medium MS + 2.4D (0,3mg/l) + CW (30%) and MS + NAA (0,5mg/l) + 2.4D (0.1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. The volume for plating was 5ml/60ml semi-solid medium. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + NAA (0,5mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). In vitro shoots of dendrobium were regeneration on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Dendrobium sp. by bioreactor technique was established to produce 6,200 plantlets per one liter of somatic embryogenesis suspension.
| Published in | American Journal of Agriculture and Forestry (Volume 10, Issue 4) |
| DOI | 10.11648/j.ajaf.20221004.12 |
| Page(s) | 131-137 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2022. Published by Science Publishing Group |
Bioreactor, Dendrobium sp., Embryonic Callus, Protocorm Like Bodies, Somatic Embryogenesis Suspension
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APA Style
Tran Van Minh. (2022). Industrial Propagation of Dendrobium sp. by Bioreactor Technique. American Journal of Agriculture and Forestry, 10(4), 131-137. https://doi.org/10.11648/j.ajaf.20221004.12
ACS Style
Tran Van Minh. Industrial Propagation of Dendrobium sp. by Bioreactor Technique. Am. J. Agric. For. 2022, 10(4), 131-137. doi: 10.11648/j.ajaf.20221004.12
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Download@article{10.11648/j.ajaf.20221004.12,
author = {Tran Van Minh},
title = {Industrial Propagation of Dendrobium sp. by Bioreactor Technique},
journal = {American Journal of Agriculture and Forestry},
volume = {10},
number = {4},
pages = {131-137},
doi = {10.11648/j.ajaf.20221004.12},
url = {https://doi.org/10.11648/j.ajaf.20221004.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20221004.12},
abstract = {Backgroud: Dendrobium sp. are production by multishoot system and it is required high of labor, energy, cost, large area. Objectives: there are needs of a new production system by using of plant cell culture techniques. Methods: manipulation of bioreactor techniques was effective ways to resolve the chalenges. Protocorm like bodies were used as planting materials. Somatic embryo callus was initiated on medium MS supplemented with 2.4D (0,3mg/l) + CW (30%). Somatic cell suspension was cultured for initiation and for proliferation and on medium MS + 2.4D (0,3mg/l) + CW (30%) and MS + NAA (0,5mg/l) + 2.4D (0.1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. The volume for plating was 5ml/60ml semi-solid medium. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + NAA (0,5mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). In vitro shoots of dendrobium were regeneration on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Dendrobium sp. by bioreactor technique was established to produce 6,200 plantlets per one liter of somatic embryogenesis suspension.},
year = {2022}
}
TY - JOUR T1 - Industrial Propagation of Dendrobium sp. by Bioreactor Technique AU - Tran Van Minh Y1 - 2022/07/22 PY - 2022 N1 - https://doi.org/10.11648/j.ajaf.20221004.12 DO - 10.11648/j.ajaf.20221004.12 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 131 EP - 137 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20221004.12 AB - Backgroud: Dendrobium sp. are production by multishoot system and it is required high of labor, energy, cost, large area. Objectives: there are needs of a new production system by using of plant cell culture techniques. Methods: manipulation of bioreactor techniques was effective ways to resolve the chalenges. Protocorm like bodies were used as planting materials. Somatic embryo callus was initiated on medium MS supplemented with 2.4D (0,3mg/l) + CW (30%). Somatic cell suspension was cultured for initiation and for proliferation and on medium MS + 2.4D (0,3mg/l) + CW (30%) and MS + NAA (0,5mg/l) + 2.4D (0.1mg/l). The volume of somatic cell suspension for bioreactor cultivation was 20%. The volume for plating was 5ml/60ml semi-solid medium. Somatic embryo suspension was cultured in bioreactor for initiation and proliferation on the medium MS + NAA (0,5mg/l) + 2.4D (1mg/l). Embryogenic suspension was stimulated on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). In vitro shoots of dendrobium were regeneration on the medium MS supplemented with BA (0.2mg/l) + NAA (0.2mg/l). Plantlets were enhanced growth and development in immersion-bioreactor cultivation by sinking/rising floated 1min/4hrs. Temperature, light intensity and stirring in stirring-bioreactor cultivation were favored at 26±2°C, 11,1-22,2μmol/m2/s, and 30rpm. Results: Micropropagation of Dendrobium sp. by bioreactor technique was established to produce 6,200 plantlets per one liter of somatic embryogenesis suspension. VL - 10 IS - 4 ER -
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