Identification of Fungi Associated to <i>Pseudips mexicanus</i> (Curculionidae: Scolytinae) as a Possible Biocontrol Agent

Isaac Tello-Salgado; Oscar Burgos-Duenas; Maria Del Rayo Sanchez-Carbente; Armando Burgos-Solorio

doi:doi:10.11648/j.ajaf.20210904.16

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Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent

Isaac Tello-Salgado, Oscar Burgos-Duenas, Maria Del Rayo Sanchez-Carbente, Armando Burgos-Solorio

Published in American Journal of Agriculture and Forestry (Volume 9, Issue 4)

Received: 6 June 2021 Accepted: 23 June 2021 Published: 13 July 2021

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Abstract

Entomopathogenic fungi are an important insect biological control group, however most of the fungi described as biological controllers have a broad spectrum of insect hosts. Pseudips mexicanus is a bark beetle that infests pine-oak forests in Mexico, causing significant economic and ecologic losses. In addition, the infestation alters the ecology of different organisms that coexist in the forest habitat. The search for bark beetle control methods other than pesticides has led to research into biological control procedures based on naturally occurring beetle pathogens. The goal of this paper was the isolation, morphological and molecular identification of fungi that naturally parasitize the bark beetle Pseudips mexicanus, as well as to carry out infection tests to propose a specific biological control alternative to this plague. Associated to this beetle, we have identified four isolates belonging to three genus: Beauveria, Lecanicillium and Trichoderma, within these some species have been used previously as biocontrollers, mainly in agricultural use, in the process of transformation of organic crops. The LVP-2 isolated, which was identified as Beauveria was tested for infection of naturally harvested bark beetles and its pathogenicity was proved, at three days post infection (dpi) most of the individuals presented little mobility of the legs and a swelling of the body was noted, however more studies are needed to determine their viability as plague controllers in Mexican forests.

Published in	American Journal of Agriculture and Forestry (Volume 9, Issue 4)
DOI	10.11648/j.ajaf.20210904.16
Page(s)	201-210
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Bark Beetle, Forest, Pine Trees, Pest Management, Biological Control

References

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Isaac Tello-Salgado, Oscar Burgos-Duenas, Maria Del Rayo Sanchez-Carbente, Armando Burgos-Solorio. (2021). Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. American Journal of Agriculture and Forestry, 9(4), 201-210. https://doi.org/10.11648/j.ajaf.20210904.16

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ACS Style

Isaac Tello-Salgado; Oscar Burgos-Duenas; Maria Del Rayo Sanchez-Carbente; Armando Burgos-Solorio. Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. Am. J. Agric. For. 2021, 9(4), 201-210. doi: 10.11648/j.ajaf.20210904.16

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AMA Style

Isaac Tello-Salgado, Oscar Burgos-Duenas, Maria Del Rayo Sanchez-Carbente, Armando Burgos-Solorio. Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. Am J Agric For. 2021;9(4):201-210. doi: 10.11648/j.ajaf.20210904.16

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@article{10.11648/j.ajaf.20210904.16,
  author = {Isaac Tello-Salgado and Oscar Burgos-Duenas and Maria Del Rayo Sanchez-Carbente and Armando Burgos-Solorio},
  title = {Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent},
  journal = {American Journal of Agriculture and Forestry},
  volume = {9},
  number = {4},
  pages = {201-210},
  doi = {10.11648/j.ajaf.20210904.16},
  url = {https://doi.org/10.11648/j.ajaf.20210904.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20210904.16},
  abstract = {Entomopathogenic fungi are an important insect biological control group, however most of the fungi described as biological controllers have a broad spectrum of insect hosts. Pseudips mexicanus is a bark beetle that infests pine-oak forests in Mexico, causing significant economic and ecologic losses. In addition, the infestation alters the ecology of different organisms that coexist in the forest habitat. The search for bark beetle control methods other than pesticides has led to research into biological control procedures based on naturally occurring beetle pathogens. The goal of this paper was the isolation, morphological and molecular identification of fungi that naturally parasitize the bark beetle Pseudips mexicanus, as well as to carry out infection tests to propose a specific biological control alternative to this plague. Associated to this beetle, we have identified four isolates belonging to three genus: Beauveria, Lecanicillium and Trichoderma, within these some species have been used previously as biocontrollers, mainly in agricultural use, in the process of transformation of organic crops. The LVP-2 isolated, which was identified as Beauveria was tested for infection of naturally harvested bark beetles and its pathogenicity was proved, at three days post infection (dpi) most of the individuals presented little mobility of the legs and a swelling of the body was noted, however more studies are needed to determine their viability as plague controllers in Mexican forests.},
 year = {2021}
}

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TY  - JOUR
T1  - Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent
AU  - Isaac Tello-Salgado
AU  - Oscar Burgos-Duenas
AU  - Maria Del Rayo Sanchez-Carbente
AU  - Armando Burgos-Solorio
Y1  - 2021/07/13
PY  - 2021
N1  - https://doi.org/10.11648/j.ajaf.20210904.16
DO  - 10.11648/j.ajaf.20210904.16
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 201
EP  - 210
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20210904.16
AB  - Entomopathogenic fungi are an important insect biological control group, however most of the fungi described as biological controllers have a broad spectrum of insect hosts. Pseudips mexicanus is a bark beetle that infests pine-oak forests in Mexico, causing significant economic and ecologic losses. In addition, the infestation alters the ecology of different organisms that coexist in the forest habitat. The search for bark beetle control methods other than pesticides has led to research into biological control procedures based on naturally occurring beetle pathogens. The goal of this paper was the isolation, morphological and molecular identification of fungi that naturally parasitize the bark beetle Pseudips mexicanus, as well as to carry out infection tests to propose a specific biological control alternative to this plague. Associated to this beetle, we have identified four isolates belonging to three genus: Beauveria, Lecanicillium and Trichoderma, within these some species have been used previously as biocontrollers, mainly in agricultural use, in the process of transformation of organic crops. The LVP-2 isolated, which was identified as Beauveria was tested for infection of naturally harvested bark beetles and its pathogenicity was proved, at three days post infection (dpi) most of the individuals presented little mobility of the legs and a swelling of the body was noted, however more studies are needed to determine their viability as plague controllers in Mexican forests.
VL  - 9
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Author Information

Isaac Tello-Salgado

Biological Research Center, Autonomous University of the State of Morelos, Cuernavaca, Mexico
Oscar Burgos-Duenas

Biological Research Center, Autonomous University of the State of Morelos, Cuernavaca, Mexico
Maria Del Rayo Sanchez-Carbente

Biotechnology Research Center, Autonomous University of the State of Morelos, Cuernavaca, Mexico
Armando Burgos-Solorio

Biological Research Center, Autonomous University of the State of Morelos, Cuernavaca, Mexico

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Isaac Tello-Salgado, Oscar Burgos-Duenas, Maria Del Rayo Sanchez-Carbente, Armando Burgos-Solorio. (2021). Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. American Journal of Agriculture and Forestry, 9(4), 201-210. https://doi.org/10.11648/j.ajaf.20210904.16

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ACS Style

Isaac Tello-Salgado; Oscar Burgos-Duenas; Maria Del Rayo Sanchez-Carbente; Armando Burgos-Solorio. Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. Am. J. Agric. For. 2021, 9(4), 201-210. doi: 10.11648/j.ajaf.20210904.16

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AMA Style

Isaac Tello-Salgado, Oscar Burgos-Duenas, Maria Del Rayo Sanchez-Carbente, Armando Burgos-Solorio. Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent. Am J Agric For. 2021;9(4):201-210. doi: 10.11648/j.ajaf.20210904.16

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@article{10.11648/j.ajaf.20210904.16,
  author = {Isaac Tello-Salgado and Oscar Burgos-Duenas and Maria Del Rayo Sanchez-Carbente and Armando Burgos-Solorio},
  title = {Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent},
  journal = {American Journal of Agriculture and Forestry},
  volume = {9},
  number = {4},
  pages = {201-210},
  doi = {10.11648/j.ajaf.20210904.16},
  url = {https://doi.org/10.11648/j.ajaf.20210904.16},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20210904.16},
  abstract = {Entomopathogenic fungi are an important insect biological control group, however most of the fungi described as biological controllers have a broad spectrum of insect hosts. Pseudips mexicanus is a bark beetle that infests pine-oak forests in Mexico, causing significant economic and ecologic losses. In addition, the infestation alters the ecology of different organisms that coexist in the forest habitat. The search for bark beetle control methods other than pesticides has led to research into biological control procedures based on naturally occurring beetle pathogens. The goal of this paper was the isolation, morphological and molecular identification of fungi that naturally parasitize the bark beetle Pseudips mexicanus, as well as to carry out infection tests to propose a specific biological control alternative to this plague. Associated to this beetle, we have identified four isolates belonging to three genus: Beauveria, Lecanicillium and Trichoderma, within these some species have been used previously as biocontrollers, mainly in agricultural use, in the process of transformation of organic crops. The LVP-2 isolated, which was identified as Beauveria was tested for infection of naturally harvested bark beetles and its pathogenicity was proved, at three days post infection (dpi) most of the individuals presented little mobility of the legs and a swelling of the body was noted, however more studies are needed to determine their viability as plague controllers in Mexican forests.},
 year = {2021}
}

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TY  - JOUR
T1  - Identification of Fungi Associated to Pseudips mexicanus (Curculionidae: Scolytinae) as a Possible Biocontrol Agent
AU  - Isaac Tello-Salgado
AU  - Oscar Burgos-Duenas
AU  - Maria Del Rayo Sanchez-Carbente
AU  - Armando Burgos-Solorio
Y1  - 2021/07/13
PY  - 2021
N1  - https://doi.org/10.11648/j.ajaf.20210904.16
DO  - 10.11648/j.ajaf.20210904.16
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 201
EP  - 210
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20210904.16
AB  - Entomopathogenic fungi are an important insect biological control group, however most of the fungi described as biological controllers have a broad spectrum of insect hosts. Pseudips mexicanus is a bark beetle that infests pine-oak forests in Mexico, causing significant economic and ecologic losses. In addition, the infestation alters the ecology of different organisms that coexist in the forest habitat. The search for bark beetle control methods other than pesticides has led to research into biological control procedures based on naturally occurring beetle pathogens. The goal of this paper was the isolation, morphological and molecular identification of fungi that naturally parasitize the bark beetle Pseudips mexicanus, as well as to carry out infection tests to propose a specific biological control alternative to this plague. Associated to this beetle, we have identified four isolates belonging to three genus: Beauveria, Lecanicillium and Trichoderma, within these some species have been used previously as biocontrollers, mainly in agricultural use, in the process of transformation of organic crops. The LVP-2 isolated, which was identified as Beauveria was tested for infection of naturally harvested bark beetles and its pathogenicity was proved, at three days post infection (dpi) most of the individuals presented little mobility of the legs and a swelling of the body was noted, however more studies are needed to determine their viability as plague controllers in Mexican forests.
VL  - 9
IS  - 4
ER  -

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