The B-Raf is the essential protein in signal pathways inside cells which is affected by cell growth direction. The B-Raf protein encoded by the BRAF gene that is located at chromosome 7, BRAF gene is also pointed out as proto-oncogene. This study aimed to detect the substitution at codon 600 causing a change of valine to glutamic acid (V600E) mutation in Iraqi females to assist its role in initiating breast cancer. Sixty biopsies tissue from breast cancer Iraqi women and 20 women with benign lesions were enrolled in this study. DNA was extracted from breast cancer biopsies samples. PCR and DNA Sequencing techniques were used to screen the BRAF V600E gene mutation as it is an essential event in the initiation of cancer. The results revealed that none Iraqi breast cancer women had BRAF V600E mutation, The annotated BRAF gene has been deposited in DDBJ/GenBank under the accession number LC547435. In conclusion: The present data indicate no BRAF V600E mutation in Iraqi breast cancer females and may not possess a role in breast cancer initiation. The current results may be refer to ineffectiveness of Vemurafenib and Encorafenib therapies that specific for patients with the BRAF V600 mutation. Other studies with large numbers of patients are needed to confirm the result of this study, as the high prevalence of breast cancer among Iraqi women.
| Published in | Advances in Bioscience and Bioengineering (Volume 12, Issue 2) |
| DOI | 10.11648/j.abb.20241202.13 |
| Page(s) | 45-49 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
BRAF V600E, Breast, Cancer, BRAF Gene, Iraq
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APA Style
Mahood, W. S., Hassoon, A. H., Obaidy, S. S. A., Ahmed, L. Q., Musawi, I. H. A. (2024). Investigation of BRAF V600E Mutation in Breast Cancer Patients. Advances in Bioscience and Bioengineering, 12(2), 45-49. https://doi.org/10.11648/j.abb.20241202.13
ACS Style
Mahood, W. S.; Hassoon, A. H.; Obaidy, S. S. A.; Ahmed, L. Q.; Musawi, I. H. A. Investigation of BRAF V600E Mutation in Breast Cancer Patients. Adv. BioSci. Bioeng. 2024, 12(2), 45-49. doi: 10.11648/j.abb.20241202.13
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Download@article{10.11648/j.abb.20241202.13,
author = {Wafaa Sabri Mahood and Athraa Hammed Hassoon and Shaimaa Sabah Al Obaidy and Lenha Qutaiba Ahmed and Ibtisam Hammood AL Musawi},
title = {Investigation of BRAF V600E Mutation in Breast Cancer Patients
},
journal = {Advances in Bioscience and Bioengineering},
volume = {12},
number = {2},
pages = {45-49},
doi = {10.11648/j.abb.20241202.13},
url = {https://doi.org/10.11648/j.abb.20241202.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.abb.20241202.13},
abstract = {The B-Raf is the essential protein in signal pathways inside cells which is affected by cell growth direction. The B-Raf protein encoded by the BRAF gene that is located at chromosome 7, BRAF gene is also pointed out as proto-oncogene. This study aimed to detect the substitution at codon 600 causing a change of valine to glutamic acid (V600E) mutation in Iraqi females to assist its role in initiating breast cancer. Sixty biopsies tissue from breast cancer Iraqi women and 20 women with benign lesions were enrolled in this study. DNA was extracted from breast cancer biopsies samples. PCR and DNA Sequencing techniques were used to screen the BRAF V600E gene mutation as it is an essential event in the initiation of cancer. The results revealed that none Iraqi breast cancer women had BRAF V600E mutation, The annotated BRAF gene has been deposited in DDBJ/GenBank under the accession number LC547435. In conclusion: The present data indicate no BRAF V600E mutation in Iraqi breast cancer females and may not possess a role in breast cancer initiation. The current results may be refer to ineffectiveness of Vemurafenib and Encorafenib therapies that specific for patients with the BRAF V600 mutation. Other studies with large numbers of patients are needed to confirm the result of this study, as the high prevalence of breast cancer among Iraqi women.
},
year = {2024}
}
TY - JOUR T1 - Investigation of BRAF V600E Mutation in Breast Cancer Patients AU - Wafaa Sabri Mahood AU - Athraa Hammed Hassoon AU - Shaimaa Sabah Al Obaidy AU - Lenha Qutaiba Ahmed AU - Ibtisam Hammood AL Musawi Y1 - 2024/06/19 PY - 2024 N1 - https://doi.org/10.11648/j.abb.20241202.13 DO - 10.11648/j.abb.20241202.13 T2 - Advances in Bioscience and Bioengineering JF - Advances in Bioscience and Bioengineering JO - Advances in Bioscience and Bioengineering SP - 45 EP - 49 PB - Science Publishing Group SN - 2330-4162 UR - https://doi.org/10.11648/j.abb.20241202.13 AB - The B-Raf is the essential protein in signal pathways inside cells which is affected by cell growth direction. The B-Raf protein encoded by the BRAF gene that is located at chromosome 7, BRAF gene is also pointed out as proto-oncogene. This study aimed to detect the substitution at codon 600 causing a change of valine to glutamic acid (V600E) mutation in Iraqi females to assist its role in initiating breast cancer. Sixty biopsies tissue from breast cancer Iraqi women and 20 women with benign lesions were enrolled in this study. DNA was extracted from breast cancer biopsies samples. PCR and DNA Sequencing techniques were used to screen the BRAF V600E gene mutation as it is an essential event in the initiation of cancer. The results revealed that none Iraqi breast cancer women had BRAF V600E mutation, The annotated BRAF gene has been deposited in DDBJ/GenBank under the accession number LC547435. In conclusion: The present data indicate no BRAF V600E mutation in Iraqi breast cancer females and may not possess a role in breast cancer initiation. The current results may be refer to ineffectiveness of Vemurafenib and Encorafenib therapies that specific for patients with the BRAF V600 mutation. Other studies with large numbers of patients are needed to confirm the result of this study, as the high prevalence of breast cancer among Iraqi women. VL - 12 IS - 2 ER -
College of Education- for Pure Sciences Ibn Al-Haytham, University of Baghdad, Baghdad, Iraq
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