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Efficacy of Specially Formulated Combination Three Probiotic Strains with Vitamin D3 and Zn in Children with Atopic Dermatitis

Introduction: Atopic dermatitis (AD) is a chronic inflammatory, pruritic and relapsing skin disease, frequently originated in infants and young children. The objective of our study was to evaluate the clinical efficacy and quality of life in children with AD who were treated with mixture of 3 x 109 CFU of 3 probiotic strains Lactobacillus Casei BL 2401, Lactobacillus Salivarius BL 2201, Bifidobacterium Breve BL 3406 in combination with 7mg Zinc in Hypro-ri form for better resorption and 1200 IJ Vitamin D3, once per day for 3 months. Method: This prospective case control study was conducted in the University Hospital “Dr Dragiša Mišović”, Belgrade, Serbia, Hospital for Children’s Hospital for Lung diseases and Tb. The study enrolled 150 patients with AD aged between 3 and 17 years. 75 participants received the treatment: mixture of 3 x 109 CFU of 3 probiotic strains Lactobacillus Casei BL 2401, Lactobacillus Salivarius BL 2201, Bifidobacterium Breve BL 3406 in combination with 7mg Zinc in Hypro-ri form for better resorption and 1200 IJ Vitamin D3, once per day for 3 months as an add-on to standard AD treatment and 75 of them were treated according to standard protocols for AD. Results: There is a statistically significant difference in SCORAD value before and after the treatment between the groups. Discussion: Our study was the first that assess the efficacy of a mixture of 3 x 109 CFU probiotic strains Lactobacillus Casei BL 2401, Lactobacillus Salivarius BL 2201, Bifidobacterium Breve BL 3406 in combination with 7mg Zinc in Hypro-ri® form for better resorption and 1200 IJ Vitamin D3 in children with atopic dermatitis. Our study confirmed that regular treatment with this specific probiotic mixture significantly decreased pruritus and sleep problems and improved quality of life in highly atopic children with severe atopic dermatitis.

Children, Atopic Dermatitis, Probiotics

APA Style

Ivana Filipovic, Olivera Ostojic, Vesna Vekovic, Sladjana Mihajlovic, Marco Caminati, et al. (2023). Efficacy of Specially Formulated Combination Three Probiotic Strains with Vitamin D3 and Zn in Children with Atopic Dermatitis. American Journal of Pediatrics, 9(4), 186-192. https://doi.org/10.11648/j.ajp.20230904.11

ACS Style

Ivana Filipovic; Olivera Ostojic; Vesna Vekovic; Sladjana Mihajlovic; Marco Caminati, et al. Efficacy of Specially Formulated Combination Three Probiotic Strains with Vitamin D3 and Zn in Children with Atopic Dermatitis. Am. J. Pediatr. 2023, 9(4), 186-192. doi: 10.11648/j.ajp.20230904.11

AMA Style

Ivana Filipovic, Olivera Ostojic, Vesna Vekovic, Sladjana Mihajlovic, Marco Caminati, et al. Efficacy of Specially Formulated Combination Three Probiotic Strains with Vitamin D3 and Zn in Children with Atopic Dermatitis. Am J Pediatr. 2023;9(4):186-192. doi: 10.11648/j.ajp.20230904.11

Copyright © 2023 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

1. Ständer S. Atopic dermatitis. N Engl J Med. 2021; 384 (12): 1136-43.
2. Kim J, Kim BE, Leung DYM. Pathophysiology of atopic dermatitis: Clinical implications. Allergy Asthma Proc. 2019; 40 (2): 84-92.
3. Weidinger, S.; Beck, L. A.; Bieber, T.; Kabashima, K.; Irvine, A. D. Atopic dermatitis. Nat. Rev. Dis. Primers 2018, 4, 1.
4. Ziyab AH, Ewart S, Lockett GA, Zhang H, Arshad H, Holloway JW, Karmaus W. Expression of the filaggrin gene in umbilical cord blood predicts eczema risk in infancy: A birth cohort study. Clin Exp Allergy. 2017 Sep; 47 (9): 1185-1192.
5. Suzuki, H.; Makino, Y.; Nagata, M.; Furuta, J.; Enomoto, H.; Hirota, T.; Tamari, M.; Noguchi, E. A rare variant in CYP27A1 and its association with atopic dermatitis with high serum total IgE. Allergy 2016, 71, 1486–1489.
6. Bjerre, R.; Bandier, J.; Skov, L.; Engstrand, L.; Johansen, J. D. The role of the skin microbiome in atopic dermatitis: A systematic review. Br. J. Dermatol. 2017, 177, 1272–1278.
7. Brauweiler, A. M.; Hall, C. F.; Goleva, E.; Leung, D. Y. Staphylococcus aureus Lipoteichoic Acid Inhibits Keratinocyte Differentiation through a p63-Mediated Pathway. J. Investig. Dermatol. 2017, 137, 2030–2033.
8. Arkwright PD, Koplin JJ. Impact of a decade of research into atopic dermatitis. J Allergy Clin Immunol Pract. 2023; 11 (1): 63-71.
9. Fang, Z.; Li, L.; Zhang, H.; Zhao, J.; Lu, W.; Chen, W. Gut Microbiota, Probiotics, and Their Interactions in Prevention and Treatment of Atopic Dermatitis: A Review. Front. Immunol. 2021, 12, 720393.
10. de Goffau MC, Lager S, Sovio U, Gaccioli F, Cook E, Peacock SJ, Parkhill J, Charnock-Jones DS, Smith GCS. Human placenta has no microbiome but can contain potential pathogens. Nature. 2019 Aug; 572 (7769): 329-334.
11. Milani, C.; Duranti, S.; Bottacini, F.; Casey, E.; Turroni, F.; Mahony, J.; Belzer, C.; Delgado Palacio, S.; Arboleya Montes, S.; Mancabelli, L.; et al. The First Microbial Colonizers of the Human Gut: Composition, Activities, and Health Implications of the Infant Gut Microbiota. Microbiol. Mol. Biol. Rev. 2017, 81, e00036-17.
12. Jakobsson, H. E.; Abrahamsson, T. R.; Jenmalm, M. C.; Harris, K.; Quince, C.; Jernberg, C.; Björkstén, B.; Engstrand, L.; Andersson, A. F. Decreased gut microbiota diversity, delayed Bacteroidetes colonisation and reduced Th1 responses in infants delivered by Caesarean section. Gut 2014, 63, 559–566.
13. Brumbaugh, D. E.; Arruda, J.; Robbins, K.; Ir, D.; Santorico, S. A.; Robertson, C. E.; Frank, D. N. Mode of Delivery Determines Neonatal Pharyngeal Bacterial Composition and Early Intestinal Colonization. J. Pediatr. Gastroenterol. Nutr. 2016, 63, 320–328.
14. Francino, M. P. Birth Mode-Related Differences in Gut Microbiota Colonization and Immune System Development. Ann. Nutr. Metab. 2018, 73 (Suppl. 3), 12–16.
15. Neri I, Galli E, Baiardini I, Picozza M, Rossi AB, Matruglio P, et al. Implications of atopic dermatitis on the quality of life of 6-11 years old children and caregivers (PEDI-BURDEN). J Asthma Allergy. 2023; 16: 383-96.
16. Fasseeh AN, Elezbawy B, Korra N, Tannira M, Dalle H, Aderian S, et al. Burden of atopic dermatitis in adults and adolescents: a systematic literature review. Dermatol Ther (Heidelb). 2022; 12 (12): 2653-68.
17. Bieber T. Atopic dermatitis: an expanding therapeutic pipeline for a complex disease. Nat Rev Drug Discov. 2022; 21 (1): 21-40.
18. Wang F, Wu F, Chen H, Tang B. The effect of probiotics in the prevention of atopic dermatitis in children: a systematic review and meta-analysis. Transl Pediatr. 2023; 12 (4): 731-48.
19. Fijan S, Kolč N, Hrašovec M, Jamtvedt G, Pogačar MŠ, Mičetić Turk D, Maver U. Single-strain probiotic Lactobacilli for the treatment of atopic dermatitis in children: A systematic review and meta-analysis. Pharmaceutics. 2023; 15 (4): 1256.
20. Umborowati MA, Damayanti D, Anggraeni S, Endaryanto A, Surono IS, Effendy I, Prakoeswa CRS. The role of probiotics in the treatment of adult atopic dermatitis: a meta-analysis of randomized controlled trials. J Health Popul Nutr. 2022; 41 (1): 37.
21. Li Y, Zhang B, Guo J, Cao Z, Shen M. The efficacy of probiotics supplementation for the treatment of atopic dermatitis in adults: a systematic review and meta-analysis. J Dermatolog Treat. 2022; 33 (6): 2800-9.
22. Fiocchi, A.; Pawankar, R.; Cuello-Garcia, C.; Ahn, K.; Al- Hammadi, S.; Agarwal, A.; Beyer, K.; Burks, W.; Canonica, G. W.; Ebisawa, M.; et al. World allergy organization- mcmaster university guidelines for allergic disease prevention (GLAD-P): Probiotics. World Allergy Organ. J. 2015, 27.
23. Filipovic I, Ostojic O, Vekovic V, Lackovic M, Zivkovic Z, Combination of Lactobacillus Rhamnosus LGG, Vitamin D3 and Zn in Preventing Atopic Dermatitis in Infancy, American Journal of Pediatrics. 2020; 6 (3): 280-284. doi: 10.11648/j.ajp.20200603.26.
24. Zivkovic Z, VekovicV, Jocic-Stojanovi J, Filipovic I, Caminati M, Korcok D, Mitrovic S and Ostojic O. Visual Analog Scale-An easy Method of Cough Assessment in Children. EC Pulmonology and Respiratory Medicine 2019; 8.11: 82-91.
25. Filipovic I, Lackovic M, Mihajlovic M, Bakic T, Filipovic Đ and Zivkovic Z. Probiotics in allergic diseases. In the book: Atopic Dermatitis (ISBN 978-1-83962-724-8) InTech, Published: September 16th 2020. DOI: 10.5772/intechopen.93535.
26. I. Filipović, M. Lacković, I. Plješa, S. Mihajlović, Z. Živković,, et at Uloga probiotika u primarnoj prevenciji atopijskih bolesti - Kad je pravo vreme? Prev Ped, 2021; 7 (1-2): 27–30.